Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab9053
• Prices: $403.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ChIP ChIP IHC-P Electron microscopy IHC-P IHC-F ICC/IF ICC/IF FC WB ChIP
• Species Reactivities: Mouse, Rat, Bovine, Human, S. pombe, Toxoplasma gondii, Mammalian
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H4, tri methylated at K20
• Blocking Peptide: Human Histone H4 (tri methyl K20) peptide
• Description: Rabbit Polyclonal
• Gene: HIST4H4
• Conjugate: Unconjugated

Product images

Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The  ChIP was performed with 25 µg of chromatin, 6 µl of  ab9053 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

All lanes : Anti-Histone H4 (tri methyl K20) antibody - ChIP Grade (ab9053) at 1 µg/ml (Calf thymus histone lysate)Lane 1 : Human Histone H4 peptide (ab2622)Lane 2 : Human Histone H4 peptide (ab2622)Lane 3 : Human Histone H4 (mono methyl K20) peptide (ab17043)Lane 4 : Human Histone H4 (di methyl K20) peptide (ab14964)Lane 5 : Human Histone H4 (tri methyl K20) peptide (ab17567)Lane 6 : Human Histone H3 (tri methyl K4) peptide (ab1342)Lane 7 : Human Histone H3 (tri methyl K9) peptide (ab1773)Blocking peptides at 1 µg/ml per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

IHC image of Histone H4 (tri methyl K20) staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9053, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

HeLa cells immunofluorescently stained with anti-Tri Methyl Lysine 20 of histone H4 (ab9053). HeLa cells were cultured on glass coverslips, fixed with 4% paraformaldehyde, and immunofluorescently labeled with anti-Tri K20 (green) at 1/500 and DAPI (red). This image is part of the nuclear antibody characterisation program at www.cellnucleus.comThe image was collected using a 1.3 N.A. 40 X PlanApo Objective (Zeiss) using a Zeiss Axiovert 200M inverted fluorescent microscope and a SensiCam HQ (Cooke Corp.) 12-bit CCD. The images were collected using Universal Imaging MetaMorph v. 5.1 for Windows. A Physik Instruments piezo z-drive was used to collect images at 200 nm intervals throughout the depth of the cell nucleus. The digital images were processed to remove fluorescent background and colour composites were made from single focal planes using MetaMorph

A mouse 10T1/2 embryonic fibroblast immunofluorescently stained with anti Tri Methyl Lysine 20 of histone H4. Murine 10T1/2 embryonic fibroblasts were cultured on glass coverslips, fixed with paraformaldehyde and then immunofluorescently labeled using an anti-Tri Methyl K20 of histone H4 antibody at 1/500 (green). The nuclear chromatin was simultaneously visualized using DAPI (red). This image is part of the nuclear antibody characterisation program at www.cellnucleus.comThe image was collected using a 1.4 N.A. 100 X PlanApo Objective (Zeiss) using a Zeiss Axiovert 200M inverted fluorescent microscope and a Photometrics Cascade 16-bit CCD. The images were collected using Universal Imaging MetaMorph v. 5.1 for Windows. A Physik Instruments piezo z-drive was used to collect images at 200 nm intervals throughout the depth of the cell nucleus. The digital images were processed to remove fluorescent background and colour com

A field of HeLa cells stained with anti-Trimethy Lysine 20 of Histone H4. This image is part of the nuclear antibody characterisation program at www.cellnucleus.com

Product References

Targeting and tracing of specific DNA sequences with dTALEs in living cells. - Targeting and tracing of specific DNA sequences with dTALEs in living cells.

Thanisch K, Schneider K, Morbitzer R, Solovei I, Lahaye T, Bultmann S, Leonhardt H. Nucleic Acids Res. 2014 Apr;42(6):e38.

Small RNA profiling of Xenopus embryos reveals novel miRNAs and a new class of - Small RNA profiling of Xenopus embryos reveals novel miRNAs and a new class of

Harding JL, Horswell S, Heliot C, Armisen J, Zimmerman LB, Luscombe NM, Miska EA, Hill CS. Genome Res. 2014 Jan;24(1):96-106.

Radiation-induced alterations of histone post-translational modification levels - Radiation-induced alterations of histone post-translational modification levels

Maroschik B, Gurtler A, Kramer A, Rossler U, Gomolka M, Hornhardt S, Mortl S, Friedl AA. Radiat Oncol. 2014 Jan 9;9:15.

Dynamics of the two heterochromatin types during imprinted X chromosome - Dynamics of the two heterochromatin types during imprinted X chromosome

Vaskova EA, Dementyeva EV, Shevchenko AI, Pavlova SV, Grigor'eva EV, Zhelezova AI, Vandeberg JL, Zakian SM. PLoS One. 2014 Feb 4;9(2):e88256.

Nucleosome acidic patch promotes RNF168- and RING1B/BMI1-dependent H2AX and H2A - Nucleosome acidic patch promotes RNF168- and RING1B/BMI1-dependent H2AX and H2A

Leung JW, Agarwal P, Canny MD, Gong F, Robison AD, Finkelstein IJ, Durocher D, Miller KM. PLoS Genet. 2014 Mar 6;10(3):e1004178.

A dual role for the histone methyltransferase PR-SET7/SETD8 and histone H4 lysine - A dual role for the histone methyltransferase PR-SET7/SETD8 and histone H4 lysine

Kapoor-Vazirani P, Vertino PM. J Biol Chem. 2014 Mar 14;289(11):7425-37.

Blimp-1 represses CD8 T cell expression of PD-1 using a feed-forward - Blimp-1 represses CD8 T cell expression of PD-1 using a feed-forward

Lu P, Youngblood BA, Austin JW, Mohammed AU, Butler R, Ahmed R, Boss JM. J Exp Med. 2014 Mar 10;211(3):515-27.

The maternal nucleolus plays a key role in centromere satellite maintenance - The maternal nucleolus plays a key role in centromere satellite maintenance

Fulka H, Langerova A. Development. 2014 Apr;141(8):1694-704.

Elucidating the molecular bases of epigenetic inheritance in non-model - Elucidating the molecular bases of epigenetic inheritance in non-model

Perfus-Barbeoch L, Castagnone-Sereno P, Reichelt M, Fneich S, Roquis D, Pratx L, Cosseau C, Grunau C, Abad P. Front Physiol. 2014 Jun 6;5:211.

Variant PRC1 complex-dependent H2A ubiquitylation drives PRC2 recruitment and - Variant PRC1 complex-dependent H2A ubiquitylation drives PRC2 recruitment and

Blackledge NP, Farcas AM, Kondo T, King HW, McGouran JF, Hanssen LL, Ito S, Cooper S, Kondo K, Koseki Y, Ishikura T, Long HK, Sheahan TW, Brockdorff N, Kessler BM, Koseki H, Klose RJ. Cell. 2014 Jun 5;157(6):1445-59.