Anti-HLA G antibody [G233]

• Supplier: Abcam
• Catalog: ab52454
• Prices: $384.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: G233
• Applications: ICC/IF ICC/IF IP ELISA FC
• Species Reactivities: Human
• Antigen: Murine L cells transfected with both human beta2-microglobulin and HLA-G
• Description: Mouse Monoclonal
• Gene: HLA-G
• Conjugate: Unconjugated

Product images

ICC/IF image of ab52454 stained Jeg3 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52454, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

The C-terminal amino acid of the MHC-I heavy chain is critical for binding to - The C-terminal amino acid of the MHC-I heavy chain is critical for binding to

Cho S, Kim BY, Ahn K, Jun Y. PLoS One. 2013 Aug 12;8(8):e72356.

Identification of circulating nonclassic human leukocyte antigen G (HLA-G)-like - Identification of circulating nonclassic human leukocyte antigen G (HLA-G)-like

Gonzalez A, Alegre E, Arroyo A, LeMaoult J, Echeveste JI. Clin Chem. 2011 Jul;57(7):1013-22.

Retrieval of trophoblast cells from the cervical canal for prediction of abnormal - Retrieval of trophoblast cells from the cervical canal for prediction of abnormal

Imudia AN, Suzuki Y, Kilburn BA, Yelian FD, Diamond MP, Romero R, Armant DR. Hum Reprod. 2009 Sep;24(9):2086-92.

HLA-G positive trophoblastic cells in transcervical samples and their isolation - HLA-G positive trophoblastic cells in transcervical samples and their isolation

Bulmer JN, Cioni R, Bussani C, Cirigliano V, Sole F, Costa C, Garcia P, Adinolfi M. Prenat Diagn. 2003 Jan;23(1):34-9.

Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot - Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot

Frumento G, Franchello S, Palmisano GL, Nicotra MR, Giacomini P, Loke YW, Geraghty DE, Maio M, Manzo C, Natali PG, Ferrara GB. Tissue Antigens. 2000 Jul;56(1):30-7.

A human histocompatibility leukocyte antigen (HLA)-G-specific receptor expressed - A human histocompatibility leukocyte antigen (HLA)-G-specific receptor expressed

Rajagopalan S, Long EO. J Exp Med. 1999 Apr 5;189(7):1093-100.