Anti-HMW Cytokeratin antibody [34betaE12]
| Name | Anti-HMW Cytokeratin antibody [34betaE12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab86725 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 34betaE12 |
| Applications | IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Dog, Human |
| Antigen | HMW CK 34betaE12 |
| Description | Mouse Monoclonal |
| Gene | KRT13 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab86725, at 1/50 dilution, staining Human HMW Cytokeratin in prostate, using Immunohistochemistry, Formalin/PFA-fixed paraffin embedded tissue.
![Overlay histogram showing A431 cells stained with ab86725 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86725, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3089_HMW-Cytokeratin-Primary-antibodies-ab86725-2.jpg)
Overlay histogram showing A431 cells stained with ab86725 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86725, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ICC/IF image of ab86725 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86725 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Use of keratin 35betaE12 as an adjunct in the diagnosis of mammary - Use of keratin 35betaE12 as an adjunct in the diagnosis of mammary
Moinfar F, Man YG, Lininger RA, Bodian C, Tavassoli FA. Am J Surg Pathol. 1999 Sep;23(9):1048-58.
Effect of formalin fixation and epitope retrieval techniques on antibody - Effect of formalin fixation and epitope retrieval techniques on antibody
Varma M, Linden MD, Amin MB. Mod Pathol. 1999 May;12(5):472-8.
Steam heat with an EDTA buffer and protease digestion optimizes - Steam heat with an EDTA buffer and protease digestion optimizes
Iczkowski KA, Cheng L, Crawford BG, Bostwick DG. Mod Pathol. 1999 Jan;12(1):1-4.
Distinction of basaloid squamous cell carcinoma from adenoid cystic and small - Distinction of basaloid squamous cell carcinoma from adenoid cystic and small
Morice WG, Ferreiro JA. Hum Pathol. 1998 Jun;29(6):609-12.