Anti-HNMT antibody - Aminoterminal end
| Name | Anti-HNMT antibody - Aminoterminal end |
|---|---|
| Supplier | Abcam |
| Catalog | ab63147 |
| Prices | $359.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide (Human) conjugated to KLH of the N terminal region of human HNMT |
| Description | Rabbit Polyclonal |
| Gene | HNMT |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab63147 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63147, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-HNMT antibody - Aminoterminal end (ab63147) at 1/50 dilution + Hela cell line lysates at 35 µg
Immunohistochemical analysis of HNMT expression in paraffin embedded formalin fixed human hepatocarcinoma tissue using 1/10 ab63147, followed by peroxidase-conjugated secondary antibody step and DAB staining.
Product References
Histamine transport and metabolism are deranged in salivary glands in Sjogren's - Histamine transport and metabolism are deranged in salivary glands in Sjogren's
Stegaev V, Nies AT, Porola P, Mieliauskaite D, Sanchez-Jimenez F, Urdiales JL, Sillat T, Schwelberger HG, Chazot PL, Katebe M, Mackiewicz Z, Konttinen YT, Nordstrom DC. Rheumatology (Oxford). 2013 Sep;52(9):1599-608.
Differences in the expression of histamine-related genes and proteins in normal - Differences in the expression of histamine-related genes and proteins in normal
Szabo PM, Wiener Z, Tombol Z, Kovacs A, Pocza P, Horanyi J, Kulka J, Riesz P, Toth M, Patocs A, Gaillard RC, Falus A, Racz K, Igaz P. Virchows Arch. 2009 Aug;455(2):133-42.