Anti-hnRNP A1 antibody
| Name | Anti-hnRNP A1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab4791 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IP ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat, Monkey |
| Antigen | Synthetic peptide derived from residues 300 to the C-terminus of Human hnRNP A1 |
| Blocking Peptide | hnRNP A1 peptide |
| Description | Rabbit Polyclonal |
| Gene | HNRNPA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-hnRNP A1 antibody (ab4791) at 1/500 dilutionLane 1 : HeLa Whole Cell ExtractLane 2 : HeLa Nuclear ExtractSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
![hnRNP A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to hnRNP A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab4791.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 36kDa: hnRNP A1.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3230_hnRNP-A1-Primary-antibodies-ab4791-11.jpg)
hnRNP A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to hnRNP A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab4791.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 36kDa: hnRNP A1.
Immunofluorescent imaging of human cells (U2OS) with ab4791 reveals the expected ribonucleoprotein particular staining in the nucleus. IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/200 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. Nuclei counterstained with Hoechst stain (blue).
IHC image of hnRNP A1 staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4791, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product References
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