Anti-hnRNP A2B1 antibody
| Name | Anti-hnRNP A2B1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31645 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Human, Chicken, Dog, Xenopus |
| Antigen | Synthetic peptide derived from within residues 50 - 150 of Human hnRNP A2B1 |
| Description | Rabbit Polyclonal |
| Gene | HNRNPA2B1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab31645 staining hnRNP A2B1 in CHO cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour 30 minutes at 21°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Green - hnRNP, Red -alpha tubulin, Blue - nuclei.See Abreview
Anti-hnRNP A2B1 antibody (ab31645) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution Performed under reducing conditions.
![hnRNP A2B1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to hnRNP A2B1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31645.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 35kDa: hnRNP A2B1.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3267_hnRNP-A2B1-Primary-antibodies-ab31645-5.jpg)
hnRNP A2B1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to hnRNP A2B1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31645.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 35kDa: hnRNP A2B1.
IHC image of ab31645 staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31645, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab31645 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab31645, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 and HepG2 cells at 5µg/ml.
Product References
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Du C, Ma X, Meruvu S, Hugendubler L, Mueller E. J Lipid Res. 2014 Sep;55(9):1886-96.
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Li X, Johansson C, Glahder J, Mossberg AK, Schwartz S. Nucleic Acids Res. 2013 Dec;41(22):10488-508.
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Bowick GC, Spratt HM, Hogg AE, Endsley JJ, Wiktorowicz JE, Kurosky A, Luxon BA, Gorenstein DG, Herzog NK. J Virol. 2009 Jan;83(2):687-700.