Immunohistochemical analysis of paraffin-embedded U373 xenograft labeling hnRNP A2B1 with ab183654 at 1/500 dilution.
All lanes : Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : HepG2 whole cell lysateLane 5 : A375 whole cell lysateLysates/proteins at 30 µg per lane.
Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution + PC-12 whole cell lysate at 30 µg
All lanes : Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilutionLane 1 : Neuro2A whole cell lysateLane 2 : GL261 whole cell lysateLane 3 : C8D30 whole cell lysateLane 4 : NIH-3T3 whole cell lysateLane 5 : BCL-1 whole cell lysateLane 6 : Raw264.7 cell lysateLane 7 : C2C12 whole cell lysateLysates/proteins at 30 µg per lane.
Western blot analysis using ab183654 at 1/200 dilution detecting hnRNP A2B1 in immunoprecipitation pellets from 4500 µg A549 whole cell lysate with ab183654 at 2 µg. Lane 2 shows Control with 2 μg of preimmune rabbit IgG.