All lanes : Anti-hnRNP F antibody (ab50982) at 1.25 µg/mlLane 1 : MW markerLane 2 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryLane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
ab50982, at a concentration of 4 µg/ml, staining hnRNP F in paraffin embedded human kidney tissue sections by Immunohistochemistry. Cells with positive label are epithelial cells of renal tubule (indicated by arrows). Magnification: 400x.
ICC/IF image of ab50982 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50982, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab50982 staining hnRNP F in primary mouse oligodendrocytes (image shows 4 days in vitro) by Immunocytochemistry/ Immunofluorescence.hnRNP F is present in the nucleus and the cytoplasm of oligodendrocytes. In addition to its prominent nuclear localization (yellow arrowheads), hnRNP F is present in granular structures throughout the cytoplasm (white arrows). Insets show enlarged areas.