Anti-hnRNP H antibody
| Name | Anti-hnRNP H antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10374 |
| Prices | $400.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP IHC-F IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Horse, Guinea Pig, Dog, Pig, Chimpanzee, Monkey, Ape, Opossum, Orangutan, Elephant |
| Antigen | Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human heterogeneous nuclear ribonucleoprotein H1 (LocusLink ID 3187) |
| Description | Rabbit Polyclonal |
| Gene | HNRNPH1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling hnRNP H with ab10374 at 1/1000 (1µg/ml). Detection: DAB.
ICC/IF image of ab10374 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10374, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab10374 staining in human normal cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10374, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
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Variations of protein levels in human amniotic fluid stem cells CD117/2 over - Variations of protein levels in human amniotic fluid stem cells CD117/2 over
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Allogenic antibody-mediated identification of head and neck cancer antigens. - Allogenic antibody-mediated identification of head and neck cancer antigens.
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