Anti-hnRNP R antibody
| Name | Anti-hnRNP R antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab30930 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Rat, Human, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human hnRNP R |
| Description | Rabbit Polyclonal |
| Gene | HNRNPR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-hnRNP R antibody (ab30930) at 1/500 dilution + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µgSecondaryIR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
All lanes : Anti-hnRNP R antibody (ab30930) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab30930 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab30930, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlas. ab30930 staining hnRNP R in human stomach, showing a strong nuclear staining pattern in all cells. Paraffin embedded human stomach tissue was incubated with ab30930 (1/1500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab30930 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
Product References
The heterogeneous nuclear ribonucleoprotein-R is necessary for axonal beta-actin - The heterogeneous nuclear ribonucleoprotein-R is necessary for axonal beta-actin
Glinka M, Herrmann T, Funk N, Havlicek S, Rossoll W, Winkler C, Sendtner M. Hum Mol Genet. 2010 May 15;19(10):1951-66.
Regulation of post-translational protein arginine methylation during HeLa cell - Regulation of post-translational protein arginine methylation during HeLa cell
Kim C, Lim Y, Yoo BC, Won NH, Kim S, Kim G. Biochim Biophys Acta. 2010 Sep;1800(9):977-85.
Proteomic identification and comparative analysis of asymmetrically - Proteomic identification and comparative analysis of asymmetrically
Lim Y, Hong E, Kwon D, Lee E. Electrophoresis. 2010 Dec;31(23-24):3823-33.
Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in - Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in
Hadian K, Vincendeau M, Mausbacher N, Nagel D, Hauck SM, Ueffing M, Loyter A, Werner T, Wolff H, Brack-Werner R. J Biol Chem. 2009 Nov 27;284(48):33384-91.