All lanes : Anti-HNRNPA0 antibody [EPR16086] (ab192251) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HNRNPA0 with ab192251 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized A549 cells labeling HNRNPA0 with ab192251 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).The two negative controls are ab192251 at 1/250 dilution, followed by Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.