Immunohistochemical analysis of formalin fixed, paraffin embedded Human cervix carcinoma labeling HNRPAB with ab174971 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunofluorescence confocal analysis of MCF7 cells labeling HNRPAB with ab174971 at 1/25 dilution. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab174971 (1/25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRPAB immunoreactivity is localized to Nucleus significantly.
Anti-HNRPAB antibody - N-terminal (ab174971) at 1/100 dilution + HepG2 cell line lysate at 35 µgSecondaryHRP anti-rabbit at 1/10000 dilutiondeveloped using the ECL technique