Anti-HOXA13 antibody
| Name | Anti-HOXA13 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab106503 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HOXA13 |
| Description | Rabbit Polyclonal |
| Gene | HOXA13 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-HOXA13 antibody (ab106503) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab52256)Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab52256) with Immunising peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of ab106503 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab106503, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.