Anti-HOXA5 antibody (ab55239) + Human skeletal muscleObserved band size : 49 kDa (why is the actual band size different from the predicted?)
Anti-HOXA5 antibody (ab55239) + Recombinant immunogen with 25 kDa tag.
All lanes : Anti-HOXA5 antibody (ab55239) at 1 µg/ml (Anti-HOXA5 Antibody )Lane 1 : HOXA5 Tranfected 293T cell lysateLane 2 : 293T cell lysateLysates/proteins at 15 µg per lane.SecondaryGoat anti-mouse IgG-HRPObserved band size : 29 kDa (why is the actual band size different from the predicted?)Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.Exposure time : 90 seconds
![Overlay histogram showing HEK293 cells stained with ab55239 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55239, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3804_HOXA5-Primary-antibodies-ab55239-17.jpg)
Overlay histogram showing HEK293 cells stained with ab55239 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55239, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.