Anti-HOXB13 antibody [EP14655] (ab192872) at 1/1000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-HOXB13 antibody [EP14655] (ab192872) at 1/1000 dilutionLane 1 : PC-3 cell lysateLane 2 : LnCaP cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling HOXB13 using ab192872 at 1/750 dilution (2 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counterstain: Hematoxylin. Negative control also shown.
Immunofluorescence analysis of PC3 cells labeling HOXB13 using ab192872 at 1/700 dilution (5 μg/ml). A Goat anti rabbit IgG (Alexa Fluor®488) at 1/400 dilution (ab150077) was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI.