All lanes : Anti-HOXB13 antibody [EPR17371] (ab201682) at 1/5000 dilutionLane 1 : Human prostate cancer lysateLane 2 : Human fetal brain lysateLane 3 : Human fetal kidney lysateLane 4 : Human fetal spleen lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-HOXB13 antibody [EPR17371] (ab201682) at 1/5000 dilutionLane 1 : LNCaP (Human prostate cancer cell line) cell lysateLane 2 : PC-3 (Human prostate cancer cell line) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human carcinoma of prostate tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human carcinoma of prostate tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human transitional cell carcinoma of bladder represents a HOXB13 negative tissue. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human cerebral cortex represents a HOXB13 negative tissue. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.