![Anti-HOXB4 antibody [EP1919Y] (ab76093) at 1/500 dilution + K562 cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/1000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/3866_ab76093.JPG)
Anti-HOXB4 antibody [EP1919Y] (ab76093) at 1/500 dilution + K562 cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded human placental tissue, using ab76093, at 1/100 dilution.
Overlay histogram showing K562 cells stained with ab76093 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76093, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.