All lanes : Anti-HOXD10 antibody (ab76897) at 1 µg/mlLane 1 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 3 : Prostate (Human) Tissue Lysate - adult normal tissue (ab30304)Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab76897 staining HOXD10 in Mouse spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/16000 in PBS + 0.1% Triton + 0.1% BSA) for 18 hours at 4°C. A Cy3®-conjugated Donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.See Abreview