All lanes : Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : MCF7 cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109028 at a dilution of 1/250.
Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling HP1 alpha (green) with ab109028 at 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/400). DAPI was used as the nuclear counterstain.Control: primary antibody (1/500) and secondary antibody Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/400).