All lanes : Anti-HSDL2 antibody [EPR13876(B)] (ab181174) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent staining of -20℃ Acetone fixed HeLa cells labeling HSDL2 using ab181174 at a 1/100 dilution and a Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at a 1/200 dilution and counterstained with Dapi.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling HSDL2 using ab181174 at a 1/100 dilution using prediluted HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.
Anti-HSDL2 antibody [EPR13876(B)] (ab181174) at 1/10000 dilution + Human fetal liver lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution