Anti-Hsp20 antibody
| Name | Anti-Hsp20 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab13491 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP IHC-P |
| Species Reactivities | Mouse, Rat, Sheep, Rabbit, Guinea Pig, Bovine, Cat, Dog, Human, Pig |
| Antigen | Rat skeletal muscle Hsp20 |
| Description | Rabbit Polyclonal |
| Gene | HSPB6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Hsp20 antibody (ab13491) at 1/2000 dilution + Lysates prepared from rat heart tissue
ICC/IF image of ab13491 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13491, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of rat ventricle tissue, staining Hsp20 with ab13491.
Product References
Mechanisms involved in increased sensitivity to adenosine A(2A) receptor - Mechanisms involved in increased sensitivity to adenosine A(2A) receptor
Hedegaard ER, Nielsen BD, Mogensen S, Rembold CM, Frobert O, Simonsen U. Eur J Pharmacol. 2014 Jan 15;723:216-26.
Transient mild hyperthermia induces E-selectin mediated localization of - Transient mild hyperthermia induces E-selectin mediated localization of
Kirui DK, Mai J, Palange AL, Qin G, van de Ven AL, Liu X, Shen H, Ferrari M. PLoS One. 2014 Feb 18;9(2):e86489.
Differential vasodilation of human placental and myometrial arteries related to - Differential vasodilation of human placental and myometrial arteries related to
Dordea AC, Sweeney M, Taggart J, Lartey J, Wessel H, Robson SC, Taggart MJ. Mol Hum Reprod. 2013 Nov;19(11):727-36.
Effects of buyang huanwu decoction on ventricular remodeling and differential - Effects of buyang huanwu decoction on ventricular remodeling and differential
Zhou YC, Liu B, Li YJ, Jing LL, Wen G, Tang J, Xu X, Lv ZP, Sun XG. Evid Based Complement Alternat Med. 2012;2012:385247.
Interactions of HSP22 (HSPB8) with HSP20, alphaB-crystallin, and HSPB3. - Interactions of HSP22 (HSPB8) with HSP20, alphaB-crystallin, and HSPB3.
Fontaine JM, Sun X, Benndorf R, Welsh MJ. Biochem Biophys Res Commun. 2005 Nov 25;337(3):1006-11. Epub 2005 Oct 3.