Anti-Hsp20 antibody [EPR14458] (ab184161) at 1/50000 dilution + Human skeletal muscle lysate at 20 µgSecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Hsp20 with ab184161 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Hsp20 with ab184161 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Hsp20 with ab184161 at 1/100 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green) followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.