All lanes : Anti-Hsp22 antibody [1E3] (ab119090) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Hsp22 cDNALysates/proteins at 5 µg per lane.developed using the ECL technique
All lanes : Anti-Hsp22 antibody [1E3] (ab119090) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.developed using the ECL technique
ab119090 at 1/100 dilution staining Hsp22 in COS7 cells transiently transfected by pCMV6-ENTRY Hsp22, by Immunofluorescence.
ab119090 at 1/100 dilution staining Hsp22 in HEK293T cells transfected with either pCMV6-ENTRY Hsp22 overexpress plasmid (Red) or empty vector control plasmid (Blue) by Flow Cytometry.
ab119090 at 1/100 dilution staining Hsp22 in Jurkat cells (Red) compared with staining with a nonspecific negative control antibody (Blue) by Flow Cytometry.