All lanes : Anti-Hsp90 beta antibody (ab196939) at 1/500 dilutionLane 1 : Extracts from HeLa cells treated with TNF alpha (20ng/ml, 30min)Lane 2 : Extracts from HeLa cells treated with TNF alpha (20ng/ml, 30min) with synthesized peptide
All lanes : Anti-Hsp90 beta antibody (ab196939) at 1/500 dilutionLane 1 : HeLa cell extract with synthesized peptideLane 2 : HeLa cell extractLane 3 : CoLo cell extractLane 4 : HepG2 cell extractLane 5 : 293 cell extract
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Hsp90 beta with ab196939 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
Immunofluorescent analysis of HeLa cells treated with TNF alpha (20nM, 15mins) labeling Hsp90 beta with ab196939 at 1/100 dilution. The image on the right is treated with the synthesized peptide.