Anti-Hsp90 beta antibody [EPR16621]

• Supplier: Abcam
• Catalog: ab203085
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16621
• Applications: WB ICC/IF ICC/IF FC IP IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment corresponding to Human Hsp90 beta aa 500 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: HSP90AB1
• Conjugate: Unconjugated

Product images

Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling Hsp90 beta with ab203085 at a 1/250 dilution showing cytoplasmic staining. Permeabilisation using 0.1% tritonX-100.Secondary ab: Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/200 dilution. Counter stain is labeling tubulin (red) with ab7291 at 1/500 dilution with secondary antibody Anti-Mouse AlexaFluor® 594 (ab150120) at 1/400 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab178854 at 1/250 dilution, Anti-Mouse AlexaFluor® 594 (ab150120) at 1/400 dilution. -ve control 2 is ab7291 at 1/500 dilution, Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/200 dilution.

All lanes : Anti-Hsp90 beta antibody [EPR16621] (ab203085) at 1/10000 dilutionLane 1 : Hsp90 alpha recombinant protein fragment (GST-tag): aa533-732Lane 2 : Hsp90 beta recombinant protein fragment (His-Tag®): aa525-724Lysates/proteins at 0.01 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated)

All lanes : Anti-Hsp90 beta antibody [EPR16621] (ab203085) at 1/20000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysatesLane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysatesLane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Hsp90 beta antibody [EPR16621] (ab203085) at 1/5000 dilutionLane 1 : Mouse brainLane 2 : Mouse heartLane 3 : Mouse kidneyLane 4 : Mouse spleenLane 5 : Rat brainLane 6 : Rat heartLane 7 : Rat kidneyLane 8 : Rat spleenLane 9 : C6 (Rat glial tumor cells) whole cell lysateLane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Hsp90 beta with ab203085 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Negative control also shown using PBS instead of primary antibody and secondary as above.

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Hsp90 beta with ab203085 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm and nucleus staining on neuron of cerebral cortex is observed. This protein is synthesized in cytoplasm, but it can shuttle between cytoplasm and nucleus depend on the cell status. Counter stained with hematoxylin. Negative control also shown using PBS instead of primary antibody and secondary as above.

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Hsp90 beta with ab203085 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm and nucleus staining on neuron of cerebral cortex is observed. This protein is synthesized in cytoplasm, but it can shuttle between cytoplasm and nucleus depending on the cell status. Counter stained with hematoxylin. Negative control also shown using PBS instead of primary antibody and secondary as above.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Hsp90 beta with ab203085 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm and nucleus staining on neuron of cerebral cortex is observed. This protein is synthesized in cytoplasm, but it can shuttle between cytoplasm and nucleus depending on the cell status. Counter stained with hematoxylin. Negative control also shown using PBS instead of primary antibody and secondary as above.

Hsp90 beta was immunoprecipitated from 1mg of Hela (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab203085 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab203085 at 1/5000 dilution. Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated, was used as secondary antibody at 1:1000 dilution.Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM/TBST.

Flow cytometry analysis of 2% paraformaldehyde fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells using ab203085 at a 1/100 dilution (red) or a Rabbit monoclonal IgG (negative) (black). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.