Anti-HSPA12A antibody [EPR16763]

• Supplier: Abcam
• Catalog: ab200838
• Prices: $99.00
• Sizes: 10 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16763
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HSPA12A aa 100-200
• Description: Rabbit Monoclonal
• Gene: HSPA12A
• Conjugate: Unconjugated

Product images

All lanes : Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/1000 dilutionLane 1 : HEK293 (Human embryonic kidney) cell lysateLane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/10000 dilution + Human fetal brain lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/1000 dilution + Human fetal kidney lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/1000 dilutionLane 1 : Mouse spleen lysateLane 2 : Rat heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human kidney tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse cerbral cortex tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat cerbral cortex tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling HSPA12A with ab200838 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on HEK293 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:--ve control 1: ab200838 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

HSPA12A was immunoprecipitated from 1mg of Human fetal brain whole cell lysate with ab200838 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200838 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human fetal brain whole cell lysate 10 µg (Input). Lane 2: ab200838 IP in Human fetal brain whole cell lysate. Lane 3 : Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200838 in Human fetal brain whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 3 minutes.