Anti-HSPA2 antibody
| Name | Anti-HSPA2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab89130 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Full length protein corresponding to amino acids 1-639 of Human HSPA2 (NP_068814 |
| Description | Mouse Polyclonal |
| Gene | HSPA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of HSPA2 staining in human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89130, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-HSPA2 antibody (ab89130) at 1 µg/ml + HeLa cell lysate at 50 µgdeveloped using the ECL technique
All lanes : Anti-HSPA2 antibody (ab89130) at 1 µg/mlLane 1 : HSPA2 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.developed using the ECL technique
ab89130 at 10 µg/ml staining HSPA2 in HeLa cells by Immunofluorescence.