All lanes : Anti-HSPC111 antibody [EPR11971] (ab174267) at 1/1000 dilutionLane 1 : Fetal liver lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : Raji cell lysateLysates/proteins at 10 µg per lane.
Flow cytometric analysis of permeabilized K562 cells using ab174267 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal liver cells lysate or (Lane 2) 1XPBS (negative control) using ab174267 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.