ab78467 staining zebrafish eye sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 10%BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 21°C, followed by staining with ab78467 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. HuC expression can be observed in amacrine and retinal ganglion cells (in green).See Abreview
Anti-HuC antibody (ab78467) at 0.5 µg/ml + rat brain tissue lysate at 10 µgSecondaryGoat anti-rabbit HRP