A titer ELISA using ab193172. The plate was coated with different amounts of human IgG3. A serial dilution of ab193172 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA of human immunoglobulins shows ab193172 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab193172 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Sandwich ELISA using ab193172 as the capture antibody (100ng/well), and a biotinylated anti human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.
Sandwich ELISA using ab193172 as the capture antibody (100ng/well), and a biotinylated anti human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.