Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling HUNK with ab181097 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with DAPI.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling HUNK with ab181097 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HUNK with ab181097 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-HUNK antibody [EPR10013] (ab181097) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : MDA-MB-435 cell lysateLane 3 : Raw264.7 cell lysateLane 4 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution