All lanes : Anti-HuR / ELAVL1 antibody (ab135740) at 1/100 dilutionLane 1 : HeLa cell line lysateLane 2 : Jurkat cell line lysateLane 3 : CEM cell line lysateLane 4 : Ramos cell line lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labelling HuR / ELAVL1 with ab135740 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells labelling HuR / ELAVL1 with ab135740 at 1/10 dilution (green) compared to negative control cells (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-HuR / ELAVL1 antibody (ab135740) at 1/100 dilutionLane 1 : Non-transfected 293 cell lysate Lane 2 : 293 cell lysate transiently transfected with the HuR / ELAVL1 gene. Lysates/proteins at 2 µg per lane.