![Overlay histogram showing MCF7 cells stained with ab110081 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110081, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5840_ab110081-6-ab110081FC.jpg)
Overlay histogram showing MCF7 cells stained with ab110081 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110081, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-HuR / ELAVL1 antibody [4G8] (ab110081) + HeLa cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5841_HuR-ELAVL1-Primary-antibodies-ab110081-1.jpg)
Anti-HuR / ELAVL1 antibody [4G8] (ab110081) + HeLa cell lysate
ab110081, at 10µg/ml, staining HuR / ELAVL1 in formalin-fixed, paraffin-embedded Human Breast tissue by Immunohistochemistry.
ab110081, at 10µg/ml, staining HuR / ELAVL1 in formalin-fixed, paraffin-embedded Human Kidney tissue by Immunohistochemistry.
ab110081, at 10µg/ml, staining HuR / ELAVL1 in formalin-fixed, paraffin-embedded Human Testis tissue by Immunohistochemistry.
ab110081, at 10µg/ml, staining HuR / ELAVL1 in HeLa cells by Immunocytochemistry.