Anti-HuR / ELAVL1 antibody [EPR17397]

• Supplier: Abcam
• Catalog: ab200342
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17397
• Applications: IHC-P WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HuR/ ELAVL1 aa 50-150
• Description: Rabbit Monoclonal
• Gene: ELAVL1
• Conjugate: Unconjugated

Product images

All lanes : Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/5000 dilutionLane 1 : 293 (Human embryonic kidney) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysateLane 4 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilution + Human fetal heart lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-HuR / ELAVL1 antibody [EPR17397] (ab200342) at 1/1000 dilutionLane 1 : Mouse heart lysateLane 2 : Mouse kidney lysateLane 3 : Rat brain lysateLane 4 : Rat spleen lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling HuR / ELAVL1 with ab200342 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on rat cerebral cortex tissue tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

HuR / ELAVL1 was immunoprecipitated from 1mg of  HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200342 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200342 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab200342 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200342 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.