Anti-HYAL2 antibody
| Name | Anti-HYAL2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab68608 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IP IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Dog, Pig, Chimpanzee |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HYAL2 |
| Description | Rabbit Polyclonal |
| Gene | HYAL2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-HYAL2 antibody (ab68608) at 1 µg/mlLane 1 : Liver (Rat) Tissue LysateLane 2 : Liver (Mouse) Tissue LysateLane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell LysateLane 4 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 7 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate - tumor cell line (ab30124)Lane 8 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab68608 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68608, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Product References
Hyaluronan accumulation and arrested oligodendrocyte progenitor maturation in - Hyaluronan accumulation and arrested oligodendrocyte progenitor maturation in
Bugiani M, Postma N, Polder E, Dieleman N, Scheffer PG, Sim FJ, van der Knaap MS, Boor I. Brain. 2013 Jan;136(Pt 1):209-22.
Inverse expression of hyaluronidase 2 and hyaluronan synthases 1-3 is associated - Inverse expression of hyaluronidase 2 and hyaluronan synthases 1-3 is associated
Siiskonen H, Poukka M, Tyynela-Korhonen K, Sironen R, Pasonen-Seppanen S. BMC Cancer. 2013 Apr 5;13:181.
Hyal2 is a glycosylphosphatidylinositol-anchored, lipid raft-associated - Hyal2 is a glycosylphosphatidylinositol-anchored, lipid raft-associated
Andre B, Duterme C, Van Moer K, Mertens-Strijthagen J, Jadot M, Flamion B. Biochem Biophys Res Commun. 2011 Jul 22;411(1):175-9. doi: