
All lanes : Anti-HYAL2 antibody (ab66653) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Thymus (Rat) Tissue LysateLane 3 : Skeletal Muscle (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-HYAL2 antibody (ab66653) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : Rat Thymus Tissue LysateLane 4 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate - tumor cell line (ab30124)Lane 6 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.