Anti-ICAD antibody [1B10] (ab87386) at 1/500 dilution + HeLa cell lysate
Anti-ICAD antibody [1B10] (ab87386) + Human ICAD full length protein (ab87632) at 0.01 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 5000 µg/mldeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds
All lanes : Anti-ICAD antibody [1B10] (ab87386) at 1/500 dilutionLane 1 : HEK293T cells were transfected with the pCMV6-ENTRY control cDNA for 48 hrs and lysed.Lane 2 : HEK293T cells were transfected with the pCMV6-ENTRY ICAD cDNA for 48 hrs and lysed.Lysates/proteins at 5 µg per lane.
ab87386, staining ICAD in HeLa cells transiently transfected by pCMV6-ENTRY by immunofluorescence.
ab87386, staining ICAD in HeLa cells by immunofluorescence.