Anti-IER3IP1 antibody [EPR13440]

• Supplier: Abcam
• Catalog: ab181247
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13440
• Applications: FC IHC-P WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IER3IP1 aa 1 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: IER3IP1
• Conjugate: Unconjugated

Product images

All lanes : Anti-IER3IP1 antibody [EPR13440] (ab181247) at 1/1000 dilutionLane 1 : HepG2 lysateLane 2 : HeLa lysateLane 3 : Human fetal heart lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling IER3IP1 with ab181247 at 1/250 dilution, dollowed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and DAPI staining (blue).

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate  immunoprecipitated using ab181247 at 1/50 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000.

Flow Cytometricl analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling IER3IP1 with ab181247 at 1/210 (red) compared to a negative control antibody (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling IER3IP1 with ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling IER3IP1 with ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.