All lanes : Anti-IFN gamma Receptor beta antibody (ab77246) at 1 µg/mlLane 1 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 2 : Liver (Mouse) Tissue LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 4 : F9 (Mouse embryonic carcinoma cell line) Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 6 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 7 : Kidney (Mouse) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.