Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoskeleton. Recommended concentration of ab121195 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-IFT43 antibody (ab121195) at 1/250 dilutionLane 1 : Vector only transfected HEK293T lysateLane 2 : IFT43-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa)developed using the ECL technique
ab121195 at 1/50 dilution staining IFT43 in Paraffin-embedded Human testis tissue by Immunohistochemistry.