Anti-IGF1 antibody
| Name | Anti-IGF1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab9572 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB B/N ICC/IF ICC/IF ELISA |
| Species Reactivities | Mouse, Human |
| Antigen | Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1) |
| Description | Rabbit Polyclonal |
| Gene | IGF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab9572 staining Human normal liver parenchyma. Staining is localised to the cytoplasm.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab9572 staining IGF1 in mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000 in 10% goat serum) for 24 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.See Abreview
ICC/IF image of ab9572 stained human embryonic stem cells. The cells were fixed in methanol and then incubated in 10% BSA for 1 hour to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9572, 1/200) overnight at +4°C. The secondary antibody (red) was an Alexa Fluor® 568 goat anti-rabbit used at a 1/400 dilution.See Abreview
Product References
Excessive gestational weight gain and obesity contribute to altered expression of - Excessive gestational weight gain and obesity contribute to altered expression of
Ferraro ZM, Qiu Q, Gruslin A, Adamo KB. Int J Womens Health. 2013 Oct 2;5:657-65.
Regulation of cardiac microRNAs by bone marrow mononuclear cell therapy in - Regulation of cardiac microRNAs by bone marrow mononuclear cell therapy in
Iekushi K, Seeger F, Assmus B, Zeiher AM, Dimmeler S. Circulation. 2012 Apr 10;125(14):1765-73, S1-7. doi:
Characterization of the insulin-like growth factor axis in term pregnancies - Characterization of the insulin-like growth factor axis in term pregnancies
Ferraro ZM, Qiu Q, Gruslin A, Adamo KB. Hum Reprod. 2012 Aug;27(8):2467-75.
Differential regulation of igf1 and igf1r mRNA levels in the two hepatic lobes - Differential regulation of igf1 and igf1r mRNA levels in the two hepatic lobes
Darp RA, de Boo HA, Phua HH, Oliver MH, Derraik JG, Harding JE, Bloomfield FH. Reprod Fertil Dev. 2010;22(8):1188-97.
The role of IGF-1 as a major growth factor for myeloma cell lines and the - The role of IGF-1 as a major growth factor for myeloma cell lines and the
Sprynski AC, Hose D, Caillot L, Reme T, Shaughnessy JD Jr, Barlogie B, Seckinger A, Moreaux J, Hundemer M, Jourdan M, Meissner T, Jauch A, Mahtouk K, Kassambara A, Bertsch U, Rossi JF, Goldschmidt H, Klein B. Blood. 2009 May 7;113(19):4614-26.
AF4 is a critical regulator of the IGF-1 signaling pathway during Purkinje cell - AF4 is a critical regulator of the IGF-1 signaling pathway during Purkinje cell
Bitoun E, Finelli MJ, Oliver PL, Lee S, Davies KE. J Neurosci. 2009 Dec 9;29(49):15366-74.
Engineering insulin-like growth factor-1 for local delivery. - Engineering insulin-like growth factor-1 for local delivery.
Tokunou T, Miller R, Patwari P, Davis ME, Segers VF, Grodzinsky AJ, Lee RT. FASEB J. 2008 Jun;22(6):1886-93.
Amniotic IGF-I supplementation of growth-restricted fetal sheep alters IGF-I and - Amniotic IGF-I supplementation of growth-restricted fetal sheep alters IGF-I and
Shaikh S, Bloomfield FH, Bauer MK, Phua HH, Gilmour RS, Harding JE. J Endocrinol. 2005 Jul;186(1):145-55.