Anti-IGF1 Receptor antibody
| Name | Anti-IGF1 Receptor antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab90657 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat, Bovine, Pig, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human IGF1 Receptor |
| Description | Rabbit Polyclonal |
| Gene | IGF1R |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-IGF1 Receptor antibody (ab90657) at 1 µg/mlLane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab90657 stained MCF7 cells. The cells were 4% PFA fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90657, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and HepG2 cells at 5µg/ml, and in 100% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml.
Product References
Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have - Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have
Williams EC, Zhong X, Mohamed A, Li R, Liu Y, Dong Q, Ananiev GE, Mok JC, Lin BR, Lu J, Chiao C, Cherney R, Li H, Zhang SC, Chang Q. Hum Mol Genet. 2014 Jun 1;23(11):2968-80.
Effects of combined epidermal growth factor, brain-derived neurotrophic factor - Effects of combined epidermal growth factor, brain-derived neurotrophic factor
Yu Y, Yan J, Li M, Yan L, Zhao Y, Lian Y, Li R, Liu P, Qiao J. Hum Reprod. 2012 Jul;27(7):2146-59.