Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human MPC1 protein (hMPC1-DDK; +) or HA-tagged full-length human MPC2 protein (hMPC2-HA; +), using MPC1 (D2L9I) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (upper middle), HA-Tag (C29F4) Rabbit mAb #3724 (lower middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Both expression vectors were provided by Dr. Jared Rutter's laboratory at the University of Utah School of Medicine.