Anti-IGFBP2 antibody (ab91404) at 2 µg/ml + Jurkat cell lysate at 35 µg
Anti-IGFBP2 antibody (ab91404) at 1/500 dilution + Active human IGFBP2 full length protein (ab63223) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds
Formalin-fixed and paraffin-embedded human brain tissue with ab91404, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of ab91404 with A549 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of jurkat cells using ab91404 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.