Anti-IL1 beta antibody

• Supplier: Abcam
• Catalog: ab9787
• Prices: $391.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ELISA B/N IHC-F IHC-P IHC-F
• Species Reactivities: Rat
• Antigen: Highly pure (>98%) recombinant rIL-1b (rat Interleukin-1-beta)
• Description: Rabbit Polyclonal
• Gene: Il1b
• Conjugate: Unconjugated

Product images

All lanes : Anti-IL1 beta antibody (ab9787) at 0.2 µg/mlLane 1 : MultiMark MultiColor Standard (Invitrogen)Lane 2 : 250 ng/laneLane 3 : 125 ng/laneLane 4 : 62.5 ng/laneLane 5 : 31.25 ng/laneLane 6 : 15.6 ng/laneLane 7 : 7.8 ng/laneLane 8 : 3.9 ng/laneLane 9 : 1.95 ng/laneObserved band size : 17.3 kDa (why is the actual band size different from the predicted?)

Immunohistochemical analysis of rat endometrial tissue, staining IL1 beta with ab9787. Tissue was taken from pregnant rats with implantation failure. Antigen retrieval was by heat mediation in a citrate buffer (pH 6) followed by blocking with 1% BSA for 30 minutes. Sections were incubated with primary antibody (1/50) overnight at 4°C. An HRP-goat anti-rabbit IgG was used as the secondary antibody (1/200). Staining was detected using DAB.

Immunohistochemical analysis of rat brain tissue, staining IL1 beta with ab9787. Tissue was taken from rats with middle cerebral artery occlusion either untreated (left) or treated with specific MEK1/2 inhibitor U0126. Samples were fixed for 10 minutes in ice-cold acetone and then rehydrated in PBS containing 0.3% Triton X-100 for 15 minutes. The tissues were then permeabilized and blocked for 1 hour in blocking solution containing PBS, 0.3% Triton X-100, 1% BSA, and 5% normal donkey serum. Samples were incubated overnight at 4°C with primary antibody (1/400) and a Cy2-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

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