ab55275 staining IL13 rexeptor alpha 2 in Rabbit ear wound exudate tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 100% Protein block (serum free) for 30 minutes at 37°C; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1µg/ml) for 1 hour at 37°C. An undiluted HRP-conjugated Mouse anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview
IL13 receptor alpha 2 antibody (ab55275) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human stomach.
![Overlay histogram showing HeLa cells stained with ab55275 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55275, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions. Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/7506_IL13-receptor-alpha-2-Primary-antibodies-ab55275-7.jpg)
Overlay histogram showing HeLa cells stained with ab55275 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55275, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions. Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
