Sandwich ELISA analysis of Human IL17A using ab155575.Sandwich ELISA analysis of Human IL-17A monoclonal antibody was performed by coating a blank 96-well microtiter plate with 100µl per well of Human IL-17A moncolonal antibody in duplicate at 1, 3, 4, 5, 7, and 9µg/ml in DPBS and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300µl per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human IL-17A recombinant protein at 50µl per well was added in duplicate at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0 pg/ml for 2 hours at room temperature along with 50µl of Human IL-17A biotinylated monoclonal antibody (ab15575) in all applicable wells at 0.1µg/ml for 2 hours at room temperature. The plate was washed with ELISA Wash Buffer and incubated with 100µl per well of Streptavidin-HRP in all test wells at 1:40,000 dilution for 30 minutes at room temperature and then washed and incubated with 100µl per well of TMB substrate for 30 minutes at r