All lanes : Anti-IL24 antibody [EPR13281] (ab182567) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling IL24 with ab182567 at 1/30 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.
Western blot analysis of HeLa cell lysate immunoprecipitated with ab182567 at 1/50 dilution (Lane 1). Lane 2: Negative control.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.