Lane 1 : Anti-IL27 antibody - Aminoterminal end (ab62501) at 2 µg/mlLane 2 : Anti-IL27 antibody - Aminoterminal end (ab62501) at 4 µg/mlLane 1 : EL4 cell lysateLane 2 : EL4 cell lysateLysates/proteins at 15 µg per lane.SecondaryAnti-rabbit IgG
Immunocytochemistry of IL27 in EL4 cells with ab62501 antibody at 10 µg/ml.
Immunofluorescence analysis of Human dendritic cells, staining IL27 with ab62501. Cells were fixed with methanol, permeabilized with 0.1% Saponin and blocked with 4% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (10 µg/ml in blocking buffer) for 14 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody.See Abreview