Anti-IL4 antibody

• Supplier: Abcam
• Catalog: ab9622
• Prices: $384.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ELISA B/N IHC-P ELISA
• Species Reactivities: Human
• Antigen: Highly pure (>98%) recombinant hIL-4 (human Interleukin-4)
• Description: Rabbit Polyclonal
• Gene: IL4
• Conjugate: Unconjugated

Product images

ab9622 staining IL4 in human breast invasive ductal carcinoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen. Additional protein blocks were used in conjuction with secondary reagents.

Anti-IL4 antibody (ab9622) at 0.2 µg/ml + Active human IL4 full length protein (ab73186) at 0.0001 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 2 minutes

Product References

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